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KMID : 0613820110210121784
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Journal of Life Science
2011 Volume.21 No. 12 p.1784 ~ p.1788
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miR-7b Promoter Contains Negative Gene Elements
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Choi Ji-Woong
Lee Heon-Jin
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Abstract
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The typical miRNA and its nearby host gene are co-expressed by sharing the same promoter. We assumed that miR-7b and its host gene FICT might use an identical promoter for their brain specific gene expression. Sequence comparison of the genomic DNA of mouse miR-7b, human miR-7-3 and their host genes by using the bioinformatic tools revealed high sequence homology and several putative transcription factor-binding sites on the promoter region. In order to probe the hypothesis we used a luciferase vector system into which we cloned the 5' upstream conserved region of miR-7b and FICT. The putative promoter region showed decreased luciferase activity, suggesting that the 5' upstream of miR-7b and FICT contain a negative regulator for gene expression.
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KEYWORD
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miRNA, promoter, gene regulation, DNA element, cis element
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